• OH, My God, the so-called “rubber bug” is it!

     

    Black spots observed by individual experimenters in a serum-containing culture system were considered as blackworms. In fact, this dark matter may be the crystallization of protein/salt molecules in serum or culture media. The movement of this substance in the culture system is Brownian motion, which is caused by the action of the liquid. It is not the autonomous movement of living things. And no laboratory has ever separated biogenic genetic material from the black substance. As the incubation time increases at 37 ° C, the formation of precipitate increases. Proper thawing and inactivation (without heat inactivation, if not necessary) can reduce black spots. At the same time, this phenomenon usually occurs between the same batch of serum. Serum of a lot of good, pro, quickly stocking it !

    Bottle serum thaw steps

    When thawing fetal bovine serum, follow the suggested gradual thawing method (-20 ° C to 4 ° C to room temperature). Experiments have shown that it is effortless to produce precipitate if the temperature of thawed fetal bovine serum (e.g., 20 ° C to 37 ° C). Thaw fetal bovine serum, please feel free to shake evenly, so that the temperature and composition uniformity, reduce the occurrence of precipitation. Do not leave fetal bovine serum at 37 ° C for too long. If placed at 37 ℃ for too long, the fetal bovine serum will become cloudy, and many of the more unstable components of fetal bovine serum will, therefore, be compromised, and affect the quality of fetal bovine serum.

    Serum heat inactivated is necessary.

    Experiments have shown that heat-inactivated fetal bovine serum that has been appropriately processed is not required for most cells. Fetal bovine serum treated with this had little or no effect on the growth of the cells and even led to a decrease in the rate of cell growth, usually because the high-temperature treatment affected the quality of the fetal bovine serum. The heat-treated fetal calf serum, the formation of sediment will be significantly increased, the deposit observed under an inverted microscope, such as “black spots,” researchers often mistakenly believe that fetal bovine serum was contaminated, while the Fetal bovine serum placed in 37 ℃ environments, will make this precipitate more, make the researchers mistaken for the microbial expansion.

    Why do you need heat inactivation?

    Heated to inactivate the complement system, activated complement is involved in lysing cellular events, stimulating smooth muscle contraction, histamine release from cells and platelets, and activation of lymphocytes and macrophages. In immunological studies, ES cell culture, insect cells and smooth muscle cells, it is recommended to use heat-inactivated fetal bovine serum

    Black spots observed by individual experimenters in a serum-containing culture system were considered as blackworms. In fact, this dark matter may be the crystallization of protein/salt molecules in serum or culture media. The movement of this substance in the culture system is Brownian motion, which is caused by the action of the liquid. It is not the autonomous movement of living things. And no laboratory has ever separated biogenic genetic material from the black substance. As the incubation time increases at 37 ° C, the formation of precipitate increases. Proper thawing and inactivation (without heat inactivation, if not necessary) can reduce black spots. At the same time, this phenomenon usually occurs between the same batch of serum. Serum of a lot of good, pro, quickly stocking it!

    Bottle serum thaw steps

    When thawing fetal bovine serum, follow the suggested gradual thawing method (-20 ° C to 4 ° C to room temperature). Experiments have shown that it is effortless to produce precipitate if the temperature of thawed fetal bovine serum (e.g., 20 ° C to 37 ° C). Thaw fetal bovine serum, please feel free to shake evenly, so that the temperature and composition uniformity, reduce the occurrence of precipitation. Do not leave fetal bovine serum at 37 ° C for too long. If placed at 37 ℃ for too long, the fetal bovine serum will become cloudy, and many of the more unstable components of fetal bovine serum will, therefore, be compromised, and affect the quality of fetal bovine serum.

    Serum heat inactivated is necessary.

    Experiments have shown that heat-inactivated fetal bovine serum that has been appropriately processed is not required for most cells. Fetal bovine serum treated with this had little or no effect on the growth of the cells and even led to a decrease in the rate of cell growth, usually because the high-temperature treatment affected the quality of the fetal bovine serum. The heat-treated fetal calf serum, the formation of sediment will be significantly increased, the deposit observed under an inverted microscope, such as “black spots,” researchers often mistakenly believe that fetal bovine serum was contaminated, while the Fetal bovine serum placed in 37 ℃ environments, will make this precipitate more, make the researchers mistaken for the microbial expansion.

    Why do you need heat inactivation?

    Heated to inactivate the complement system, activated complement is involved in lysing cellular events, stimulating smooth muscle contraction, histamine release from cells and platelets, and activation of lymphocytes and macrophages. In immunological studies, ES cell culture, insect cells and smooth muscle cells, it is recommended to use heat-inactivated fetal bovine serum

    Read More :  OH, My God, it is “rubber bug”

    Created  by Biobool!


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